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. 2016 Apr 29;12(4):e1006021. doi: 10.1371/journal.pgen.1006021

Fig 1. Viability of cells containing the GAL-CEN3 chromosome.

Fig 1

A. Schematic of the GAL-CEN3 chromosome. The GAL-CEN3 chromosome contains a GAL1 promoter (orange box) adjacent to CEN3 on Chr 3 [2]. A LacO array was integrated approximately 3.8 kilobases from the centromere sequence of the GAL-CEN3 construct. The centroid of the LacO array is 8.8 kb from CEN3. Thick black lines represent chromosome arms. The chromosome is drawn based upon direct observations in live cells. The centromeres (red) are separated by approximately 800 nm. Cohesin (green) is enriched in the pericentromere region, about 50 kb surrounding each centromere. B. Representative cells grown on glucose or galactose. Glucose plates shown were imaged at 48 hours, galactose plates at 120 hours. C. Viability was derived from the percentage of colony forming units on galactose versus glucose. Wild type cells are the background strain not containing the GAL-CEN3 chromosome. From the left are GAL-CEN3, GAL-CEN3 mcm21Δ, GAL-CEN3 mcm21Δ sir2Δ, GAL-CEN3 mcm21Δ sir3Δ and HO-CEN3.