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. 2016 Apr 29;11(4):e0154437. doi: 10.1371/journal.pone.0154437

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© 2016 Guo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Higher magnification (20X) confocal analysis of the same regions seen in Fig 3 at 1 (A-C) and 4 (D-F) weeks. A. Three-channel (astrocyte, inflammatory cell, DAPI nuclear stain) confocal photo of D-Cy5 localization in the rat lamina 1week post-induction. Strong D-Cy5 signal is scattered within the lamina. B. Dual-channel photo showing D-Cy5 localization at high levels in astrocyte cytoplasm (arrows) in the lesioned area, revealed by Aldh1L1 immunostaining. C. Dual-channel photo showing D-Cy5 localization in inflammatory cells (arrows). D. Three-channel confocal photo of D-Cy5 localization in the rat lamina 4 weeks post-induction. D-Cy5 is reduced overall in the lesion area. E. Identification of astrocyte-specific D-Cy5 co-localization. D-Cy5 is still concentrated in astrocytes (arrows) but at considerably lower signal levels than at one week. F. Dual-channel (inflammatory cell-specific) D-Cy5 co-localization. D-Cy5 is still present in IBA1(+) cells (arrows) but at lower signal levels than at 1 week. Scale bars: 100μm.