Fig 4. Essential chemicals for CFPS using the LoFT cell extract.

The importance of each chemical for GFP expression was evaluated by systematic omission from the reaction mixtures. AA indicates the mixture of 20 amino acids. Template DNA used was 10 nM pOR2OR1-sfGFP. CFPS was performed at 29°C for 14 h. In the condition indicated by “all,” none of the chemicals were omitted. Error bars indicate standard deviations (n = 4).