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. 2016 Apr 29;11(4):e0153387. doi: 10.1371/journal.pone.0153387

Fig 1. Effects of chronic 5% and 18% CS on pulmonary EC inflammatory activation.

Fig 1

HPAEC grown on Flexcell plates were subjected to 18% or 5% CS for 48 or 72 hours. Control cells were left under static conditions. (A) ICAM1 expression was detected by western blot with corresponding antibody. β-Actin staining was used as a normalization control; n = 3, *P <0.05 vs. static. Soluble ICAM1 (B), IL-8 (C), TNFα (D) and IL-6 (E) production in stretch-preconditioned culture media was evaluated by ELISA assay; n = 4, *P<0.05 vs. 5% CS.