Skip to main content
. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604

Fig 4. Evaluation of CRISPR/Cas9 SAMs directed to Prnp.

Fig 4

(A) Relative positions of SAM sgRNA spacers with respect to the Prnp promoter region. Binding sites for AP1 and AP2 transcription factors were highlighted. (B) Schematic of the experimental workflow for SAM sgRNA evaluation. (C) Preliminary screening of SAM guides; sqRT PCR histogram shows increases in PrP protein levels for each individual SAM sgRNA tested, normalized to untransfected control levels (set to 1); assays #1 and #2 refer to independent primer pairs directed (respectively) to 5’- and 3’-proximal region of PrP mRNA.