Table 1. Putative surface-exposed proteins in salivary gland sporozoites.
| Accession Number | Description | Observed (of 6 replicates) | Cellular Function | Labeled a | SP/TM/GPI b |
|---|---|---|---|---|---|
| PF3D7_0304600 | circumsporozoite (CS) protein (CSP) | 6 | Invasion & Migration | YES | Signal, GPI |
| PF3D7_0818600 | BEM46-like protein, putative (PBLP) | 6 | Invasion & Migration | YES | TM |
| PF3D7_0104000 | thrombospondin-related sporozoite protein (TRSP) | 6 | Invasion & Migration | YES | Signal, TM |
| PF3D7_1335900 | thrombospondin-related anonymous protein (TRAP) | 6 | Invasion & Migration | YES | Signal |
| PF3D7_0919500 | sugar transporter, putative | 6 | Transporter | YES | 12 TMs |
| PF3D7_1222300 | endoplasmin, putative (GRP94) | 6 | Chaperone | YES | Signal |
| PF3D7_0511400 | conserved Plasmodium protein, unknown function | 4 | Hypothetical | YES | Signal, TM |
| PF3D7_0812300 | sporozoite surface protein 3, putative (SSP3) | 6 | Invasion & Migration | NO | Signal, TM |
| PF3D7_0204700 | hexose transporter (HT) | 6 | Transporter | NO | 12 TMs |
| PF3D7_1446900 | glutaminyl-peptide cyclotransferase, putative | 6 | Metabolism | NO | TM |
| PF3D7_1324900 | L-lactate dehydrogenase (LDH) | 6 | Metabolism | NO | TM |
| PF3D7_1133400 | apical membrane antigen 1 (AMA1) | 6 | Invasion & Migration | NO | Signal, TM |
| PF3D7_0408600 | sporozoite invasion-associated protein 1 (SIAP1) | 5 | Invasion & Migration | NO | Signal |
| PF3D7_0508000 | 6-Cys protein (P38) | 5 | Invasion & Migration | NO | Signal, GPI |
| PF3D7_0620000 | conserved Plasmodium protein, unknown function | 4 | Hypothetical | NO | Signal, GPI |
| PF3D7_0918000 | glideosome-associated protein 50 (GAP50) | 4 | Motility | NO | Signal, GPI |
| PF3D7_0917900 | heat shock protein 70 (HSP70-2) | 4 | Chaperone | NO | Signal |
| PF3D7_1406800 | glideosome associated protein with multiple membrane spans 3 (GAPM3) | 4 | Motility | NO | 6 TMs |
| PF3D7_1028900 | inner membrane complex protein 1m, putative (IMC1m) | 3 | Cytoskeleton | NO | TM |
| PF3D7_1311800 | M1-family alanyl aminopeptidase (M1AAP) | 3 | Protease | NO | TM |
| PF3D7_0828800 | GPI-anchored micronemal antigen (GAMA) | 3 | Invasion & Migration | NO | Signal, GPI |
| PF3D7_0827900 | protein disulfide isomerase (PDI8) | 3 | Chaperone | NO | Signal |
| PF3D7_1430700 | NADP-specific glutamate dehydrogenase (GDH2) | 3 | Metabolism | NO | Signal |
| PF3D7_1411100.1/2 | conserved Plasmodium membrane protein, unknown function | 3 | Hypothetical | NO | 8 TMs |
| PF3D7_0506900 | rhomboid protease ROM4 (ROM4) | 3 | Protease | NO | 6 TMs |
| PF3D7_0423500 | glideosome associated protein with multiple membrane spans 2 (GAPM2) | 3 | Motility | NO | 6 TMs |
| PF3D7_1323700 | glideosome associated protein with multiple membrane spans 1 (GAPM1) | 3 | Motility | NO | 6 TMs |
| PF3D7_1237700 | conserved protein, unknown function | 3 | Hypothetical | NO | 5 TMs |
| PF3D7_1238000 | COPI associated protein, putative | 3 | Vesicular Trafficking | NO | 4 TMs |
| PF3D7_1011500 | conserved Plasmodium membrane protein, unknown function | 3 | Hypothetical | NO | 4 TMs |
| PF3D7_1409400 | conserved Plasmodium membrane protein, unknown function | 3 | Hypothetical | NO | 4 TMs |
| PF3D7_0316700 | HVA22/TB2/DP1 family protein, putative | 3 | Hypothetical | NO | 3 TMs |
| PF3D7_1037300 | ADP/ATP transporter on adenylate translocase (ADT) | 3 | Transporter | NO | 3 TMs |
| PF3D7_0408700 | sporozoite micronemal protein essential for cell traversal (PLP1) | 3 | Invasion & Migration | NO | 2 TMs |
| PF3D7_0515700 | glideosome-associated protein 40, putative (GAP40) | 3 | Motility | NO | 10 TMs |
| PF3D7_1326000 | conserved Plasmodium protein, unknown function | 2 | Hypothetical | NO | TM |
| PF3D7_1252100 | rhoptry neck protein 3 (RON3) | 2 | Invasion & Migration | NO | Signal, 3 TMs |
| PF3D7_1457000 | signal peptide peptidase (SPP) | 2 | Signaling | NO | 8 TMs |
| PF3D7_0508200 | longevity-assurance (LAG1) protein, putative | 2 | Stress Response | NO | 7 TMs |
| PF3D7_1132800 | aquaglyceroporin (AQP) | 2 | Transporter | NO | 6 TMs, Signal |
| PF3D7_0316600 | formate-nitrite transporter (FNT) | 2 | Transporter | NO | 6 TMs |
| PF3D7_1146300 | conserved Plasmodium protein, unknown function | 2 | Hypothetical | NO | 2 TMs |
aEvidence for incorporation of the biotin label was found in the identifying mass spectra.
b SP, signal peptide; TM, transmembrane domain; GPI, glycosylphosphatidylinositol anchor