Table 2. Putative surface-exposed proteins identified from BSA- and heparin-treated salivary gland sporozoites.
| Accession Number | Description | BSA | Heparin | Untreated | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Obs. a | Enriched b | Label c | Obs. a | Enriched b | Label c | Obs. a | Enriched b | Label c | ||
| PF3D7_0304600 | circumsporozoite (CS) protein (CSP) | 3 | YES | YES | 3 | YES | YES | 6 | YES | YES |
| PF3D7_0818600 | BEM46-like protein, putative (PBLP) | 3 | YES | YES | 3 | YES | YES | 6 | YES | YES |
| PF3D7_0104000 | thrombospondin-related sporozoite protein (TRSP) | 3 | YES | YES | 3 | YES | YES | 6 | YES | YES |
| PF3D7_1446900 | glutaminyl-peptide cyclotransferase, putative | 3 | YES | YES | 3 | YES | YES | 6 | YES | - |
| PF3D7_0408600 | sporozoite invasion-associated protein 1 (SIAP1) | 3 | YES | YES | 2 | - | YES | 5 | YES | - |
| PF3D7_0812300 | sporozoite surface protein 3, putative (SSP3) | 3 | YES | YES | 3 | YES | - | 6 | YES | - |
| PF3D7_1133400 | apical membrane antigen 1 (AMA1) | 3 | YES | YES | 3 | YES | - | 6 | YES | - |
| PF3D7_0919500 | sugar transporter, putative | 3 | YES | YES | 3 | YES | - | 6 | YES | YES |
| PF3D7_0508000 | 6-Cys protein (P38) | 3 | YES | YES | 3 | YES | - | 5 | YES | - |
| PF3D7_0612800 | 6-Cys protein (P12p) | 2 | YES | YES | 1 | - | - | 1 | - | - |
| PF3D7_0104100 | conserved Plasmodium membrane protein, unknown function | 2 | YES | YES | - | - | - | - | - | - |
| PF3D7_1449000 | gamete egress and sporozoite traversal protein, putative (GEST) | 3 | - | YES | - | - | - | 3 | - | - |
| PF3D7_0620000 | conserved Plasmodium protein, unknown function | 3 | YES | - | 3 | YES | - | 4 | YES | - |
| PF3D7_1011500 | conserved Plasmodium membrane protein, unknown function | 3 | YES | - | 3 | YES | - | 3 | YES | - |
| PF3D7_1335900 | thrombospondin-related anonymous protein (TRAP) | 3 | YES | - | 3 | - | - | 6 | YES | YES |
| PF3D7_0204700 | hexose transporter (HT) | 3 | YES | - | 2 | - | - | 6 | YES | - |
| PF3D7_1222300 | endoplasmin, putative (GRP94) | 3 | YES | - | 3 | - | - | 6 | YES | YES |
| PF3D7_0506900 | rhomboid protease ROM4 (ROM4) | 3 | YES | - | 1 | - | - | 3 | YES | - |
| PF3D7_1406800 | glideosome associated protein with multiple membrane spans 3 (GAPM3) | 3 | YES | - | 1 | - | - | 4 | YES | - |
| PF3D7_0515700 | glideosome-associated protein 40, putative (GAP40) | 3 | YES | - | 1 | - | - | 3 | YES | - |
| PF3D7_1324900 | L-lactate dehydrogenase (LDH) | 3 | YES | - | - | - | - | 6 | YES | - |
| PF3D7_1452000 | rhoptry neck protein 2 (RON2) | 3 | YES | - | - | - | - | 3 | - | - |
| PF3D7_0918000 | glideosome-associated protein 50 (GAP50) | 3 | YES | - | - | - | - | 4 | YES | - |
| PF3D7_0917900 | heat shock protein 70 (HSP70-2) | 3 | YES | - | - | - | - | 4 | YES | - |
| PF3D7_1431900 | inner membrane complex suture component, putative (ISC3) | 3 | YES | - | - | - | - | 1 | - | - |
| PF3D7_0316600 | formate-nitrite transporter (FNT) | 2 | YES | - | - | - | - | 2 | YES | - |
| PF3D7_0408700 | sporozoite micronemal protein essential for cell traversal (PLP1) | 2 | YES | - | - | - | - | 3 | YES | - |
| PF3D7_0704600 | E3 ubiquitin-protein ligase (UT) | - | - | - | 1 | - | YES | 3 | - | - |
aNumber of biological replicates in which the protein was observed, out of three total (BSA- or heparin-treated) or six total (untreated).
bProtein was significantly more abundant in labeled samples compared to unlabeled controls.
cEvidence for incorporation of the biotin label was found in the identifying mass spectra.