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Effect of substrate stiffness on vimentin assembly state. Shown is the Triton X-100 soluble fraction of vimentin (black line) and fraction of cell undergoing active ruffling (red line (gray in the print version)) in human mesenchymal stem cells obtained from Lonza (Walkersville, MD) and cultured on fibronectin-coated substrates with various degrees of stiffness.
