Figure 1. Dorsal Organ Cool Cells (DOCCs) express Ir21a-Gal4.

(a) First/second instar larval anterior. Each Dorsal Organ Ganglion (grey) contains three DOCCs (blue). Anterior-Posterior axis denoted by double-headed arrow. (b,c) Ir21a-Gal4;UAS-GFP (Ir21a>GFP) labels larval DOCCs. Arrows denote cell bodies and arrowheads dendritic bulbs. (d) Temperature responses of Ir21a-Gal4;UAS-GCaMP6m-labeled DOCCs. Left panels, raw images; right panels, colors reflect fluorescence intensity. Arrows denote cell bodies. (e) Fluorescence quantified as percent change in fluorescence intensity compared to minimum intensity. n=22 cells (from 6 animals). (f,g) Temperature-responses of Ir21a-Gal4;R11F02-Gal4;UAS-GCaMP6m-labeled DOCCs. n=26 (7). Scale bars, 10 microns. Traces, average +/- SEM. Figure 1—figure supplement 1 provides an example of the 3-D imaging stacks used for calcium imaging data acquisition.

DOI: http://dx.doi.org/10.7554/eLife.13254.003

Figure 1—figure supplement 1. Larval-wide expression patterns of Ir21a-Gal4 and R11F02-Gal4.

(a) Ir21a-Gal4; UAS-GFP expression. (b) R11F02-Gal4; UAS-GFP expression. In addition to expression in the Dorsal Organ, both Gal4s exhibit expression in ~100 cells in the brain and ventral ganglion, neurons along the larval body wall and in the tail. R11F02-Gal4 is also expressed by sensory neurons in the Terminal Organ.

DOI: http://dx.doi.org/10.7554/eLife.13254.004

Figure 1—figure supplement 2. Calcium-imaging data are obtained as a three-dimensional imaging stack.

(a) Dimensions of imaging volume. DOCCs depicted in blue. (b) Maximum intensity projections used for visualizing fluorescence intensity. (c) Representative image of maximum intensity projections of Ir21a>GCaMP6m-labeled DOCCs. DOCC cell bodies remain within imaging field throughout.

DOI: http://dx.doi.org/10.7554/eLife.13254.005