Figure 2. Larval cool avoidance requires Ir21a and Ir25a.

(a) Sequence alterations in Ir21a and Ir25a alleles. Ir21a regulatory sequences present in Ir21a-Gal4 are denoted in green and regions encoding transmembrane domains (TMs) and pore region in red. Additional details provided in Figure 2—figure supplement 1. (b) Thermotaxis is quantified as navigational bias. Cool avoidance behavior was assessed by tracking larval trajectories on a ~0.36˚C/cm gradient extending from ~13.5˚C to ~21.5˚C, with a midpoint of ~17.5˚C. (c) Cool avoidance requires Ir21a and Ir25a. Ir21a>Ir21a denotes a wild type Ir21a transcript expressed under Ir21a-Gal4 control. {Ir21a⁺} and {Ir25a⁺} denote wild type genomic rescue transgenes. Letters denote statistically distinct categories (alpha=0.05; Tukey HSD). wild type, n=836 animals. Ir21a^∆1, n=74. Ir21a^∆1;Ir21a-Gal4, n=48. Ir21a^∆1;UAS-Ir21a, n=10. Ir21a^∆1;Ir21a>Ir21a, n= 88. Ir21a^∆1/ Ir21a¹²³, n=71; Ir21a^∆1/ Ir21a¹²³; {Ir21a⁺} n=70; Ir25a², n =100. Ir25a²; {Ir25a⁺} n= 247. Additional mutant analyses provided in Figure 2—figure supplement 1.

DOI: http://dx.doi.org/10.7554/eLife.13254.006

Figure 2—figure supplement 1. Structure of Ir21a locus and analysis of thermotaxis in Ir8a and Ir76b mutants.

(a) Cda5/Ir21a genomic region, denoting positions of the FRT-containing transposon insertions used to generate Ir21a^∆1 (PBc04017 and PBc02720), the sequences deleted in Ir21a^∆1, the Ir21a sequences present in the UAS-Ir21a rescue construct and the sequences present in the {Ir21⁺} genomic rescue construct. Untranslated regions are in gray. (b) Larval thermotaxis of Ir8a and Ir76b mutants quantified as navigational bias. Neither Ir8a nor Ir76b is required for cool avoidance; Ir8a mutants show enhanced cool avoidance compared to wild type. Letters denote statistically distinct categories (alpha=0.05; Tukey HSD). wild type, n=836 animals. Ir8a, n=166; Ir76b¹, n=96, Ir76b², n= 100.

DOI: http://dx.doi.org/10.7554/eLife.13254.007