Figure 6. Hot Cell-specific expression of IR21a confers cool-sensitivity upon Gr28b mutant Hot Cell neurons.

(a-c) Temperature responses of wild type (a), Gr28b mutant (b), and HC>Ir21a-expressing Gr28b mutant (c) thermoreceptors in the adult arista, monitored using HC>GCaMP6m. Dotted lines denote temperature minima. Traces, mean +/- SEM. wild type, n=11 cells (3 animals). Gr28b^Mi n=9 (3). HC>IR21a; Gr28b^Mi n=11 (3). (d) Cool responses (∆F/F_14˚C - ∆F/F_30˚C) of HC>IR21a; Gr28b^Micells were distinct from both wild type and Gr28b^Mi (p<0.01, Steel-Dwass test, letters denote statistically distinct groups).

DOI: http://dx.doi.org/10.7554/eLife.13254.013