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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 24;41:16–26. doi: 10.1016/j.dnarep.2016.03.003

Figure 3.

Figure 3

Presence of Tg in end joining products. Tg-containing or unmodified substrates were incubated for 6 hr in Bustel extracts supplemented with XLF and ddTTP as indicated. Samples were deproteinized and cut with NdeI and PstI, then denatured and annealed to 44-base complements of the expected head-to-tail ligation products and treated (or not) with EndoIII prior to denaturing gel electrophoresis. Bar graphs show the yield of 44-base products in each case (mean ± SEM for 3 independent experiments).