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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 24;41:16–26. doi: 10.1016/j.dnarep.2016.03.003

Figure 4.

Figure 4

Time course for Tg3 and Tg1 end joining and effect of dideoxynucleotides. A. and B. Tg3 was incubated in extracts containing ddTTP, ddCTP and/or XLF for the times indicated, then cut with NdeI and PstI and analyzed as in Fig. 2. One sample in (A.) was treated with EndoIII prior to NdeI/PstI cleavage, as in Fig. 2. Asterisk (*) indicates addition of a mutant XLF with an L115A mutation. C. Quantitative analysis of Tg3 ligation in the presence of dNTPs, derived from three replicates of the experiment shown in (B.). D. Time course of formation of end joining for Tg1. The Tg1 substrate was incubated in extracts, with XLF added either at the start of the reaction (●) or after 2 hr incubation (□). Reaction conditions were as in Fig. 2.