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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 24;41:16–26. doi: 10.1016/j.dnarep.2016.03.003

Figure 9.

Figure 9

Effect of 5′-proximal Tg on NHEJ. The blunt-ended substrates shown, with Tg as the second or third base from the 5′ terminus, were incubated for 6 hr in XLF-complemented Bustel extracts. A. Substrates and head-to-tail ligation products. The palindromic 36-base head-to-head product is identical to that shown in Fig. 1C, except for terminal sequence. B. Denaturing gel analysis of end joining products following cleavage with NdeI and PstI. C. Pooled data for formation of head-to-tail products, from 3 independent experiments (mean ± SEM). D. Cleavage of 5′-proximal Tg substrates by purified hNTH (mean ± SEM for 3 experiments). Substrates were incubated with the indicated concentrations of hNTH, and release of the 5′-terminal 1- or 2-base fragment was assessed by gel electrophoresis (see Supplemental Fig. 4).