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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: DNA Repair (Amst). 2016 Mar 26;41:42–53. doi: 10.1016/j.dnarep.2016.03.012

Fig. 1. Three levels of CN + H2O2-induced chromosomal fragmentation and their consequences for survival.

Fig. 1

A. The three models of ss-break-mediated double-strand DNA breaks: 1) the clustered excision model of direct breaks; 2) the replication fork collapse model of replication-dependent breaks; 3) the “DSB-behind the fork” model of segregation-dependent breaks. Parental DNA strands are in blue, while the newly-synthesized DNA strands are in red.

B. Kinetics of death of WT cells treated with varying concentrations of either CN (3 or 0.3 mM) or H2O2 (2 or 0.2 mM). Here and in the rest of the paper, all values are means of 3 or more independent measurements ± SEM.

C. A representative pulsed-field gel demonstrating chromosomal fragmentation induced in WT cells treated with 3 mM CN + 2 mM H2O2, or 0.3 mM CN + 2 mM H2O2, or 0.3 mM CN + 0.2 mM H2O2.

D. Kinetics of chromosomal fragmentation upon treatment with 3 mM CN + 2 mM H2O2, or 0.3 mM CN + 2 mM H2O2, or 0.3 mM CN + 0.2 mM H2O2 (from several gels like in “C”). Here and for the rest of the paper, fragmentation level at any time point is shown over the fragmentation level at time = 0.