A. Kinetics of survival of the recombinational repair mutants treated with 0.3 mM CN + 2 mM H2O2. The survival of 2 mM H2O2-alone treatment is shown for comparison from Fig. 4B.
B. A representative pulsed-field gel of kinetics of chromosomal fragmentation in the recA and recBC mutants treated with 0.3 mM CN + 2 mM H2O2 compared with WT and xthA mutant cells.
C. Quantification of the kinetics of chromosomal fragmentation upon treatment with 0.3 mM CN + 2 mM H2O2 in recA and recBC mutants compared to WT and xthA mutant cells (from several gels like in “B”). For comparison, fragmentation levels of H2O2-alone treatment from Fig. 4D are also shown.