Table 1.
E. coli strains and plasmids (all strains are in the BW25113 background unless indicated otherwise).
| Strain name | Relevant Genotype | Source/ CGSC# or Construction |
|---|---|---|
| AB1157 | F− λ−
rac- thi-1 hisG4 Δ(gpt-proA)62 argE3 thr-1 leuB6 kdgK51 rfbD1 araC14 lacY1 galK2 xylA5 mtl-1 tsx-33 glnV44 rpsL31. |
[87] |
| L-216 | AB1157 dnaA46(Ts) [88] lac/CE-ori::bla | Elena Kouzminova |
| L-393 | AB1157 dnaC2 (Ts) [64] | 10827 |
| AK4 | AB1157 Δ(srlR-recA306)::Tn10 | [89] |
| JB1 | AB1157 ΔrecBCD3::kan | [90] |
| N2731 | AB1157 recG258::Tn10 | [91] |
| JJC754 | AB1157 ΔruvABC232::cat | [92] |
| AK25 | AB1157 polA12(Ts)(Tn10) | Lab Collection |
| GR501 |
Hfr(PO45), λ, ligA251(ts), relA1, spoT1, thi
E1 |
6087 |
| LA20 | GR501 ligA251 ΔypeB::kan | [93] |
| SX1253 | F-, Δ(argF-lac)169, gal-490, Δ(modF- ybhJ) 803, λ[cI857 Δ(cro-bioA)], xthA791- YFP(::cat), IN(rrnD-rrnE)1, rph-1 |
12808 |
| BW535 | F-, thr-1, araC14, leuB6(Am), Δ(gpt- proA) 62, lacY1, tsx-33, glnX44(AS), galK2(Oc), λ−, Rac-0, nth- 1::kan,ble, Δ(xthA- pncA) 90, hisG4(Oc), rfbC1, mgl-51, nfo- 1::kan, rpsL31(strR),kdgK51, xylA5, mtl- 1, argE3(Oc), thiE1 |
7047 |
| N3055 | F-, λ−, IN(rrnD-rrnE)1, rph- 1, uvrA277::Tn10 |
6661 |
| JW0762-2 | ΔuvrB751::kan | 8819 |
| BW25113 | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB- 3), λ−, rph-1, Δ(rhaD-rhaB)568, hsdR514 |
[46] |
| TM21 | Δ(srlR-recA306)::Tn10 | BW25113 × P1 AK4 |
| TM22 | ΔrecBCD3::kan | BW25113 × P1 JB1 |
| TM23 | ΔruvABC232::cat | BW25113 × P1 JJC754 |
| TM24 | recG258::Tn10 ΔruvABC232::cat | TM23 × P1 N2731 |
| TM25 | xthA791-YFP(::cat) | BW25113 × P1 SX1253 |
| TM26 | xthA791-YFP(::cat) nfo-1::kan | TM25 × P1 BW535 |
| TM 27 | ligA251 ΔypeB::kan | BW25113 × P1 LA20 |
| TM 28 | polA12(Ts) (Tn10) | BW25113 × P1 AK25 |
| TM 29 | uvrA277::Tn10 | BW25113 × P1 N3055 |
| Plasmids | ||
| pCP20 | Flp recombinase gene on a temperature- sensitive replicon |
[48] |