FIG. 3.

Effect of the P-selectin patterning concentration on neutrophil rolling velocities. Mean velocities of neutrophils rolling on mP-selectin lines of different coating concentrations. Protein line patterns were formed via μFP at 16.0 dyn/cm² for 15 min. Data are shown as the average value ±SEM and were derived from three to four experiments with blood from at least three different donors. The number of cells analyzed for each point ranged from 19 to 102 cells. Statistical significance was assessed by comparing each concentration curve to the static 0.75 μg/ml curve using a two-way ANOVA with a post-hoc Sidak's multiple comparison test: * P < 0.05, ** P < 0.001, and *** P < 0.0001.