Fig. 2.

BN PAGE analysis and measurement of enzyme activities in acute liver failure in acute liver failure and biliary atresia. Acute liver failure. a Respiratory complexes isolated from control and patients with acute liver failure were separated using blue native gel electrophoresis on gradient Bis-Tris acrylamide gels. b Quantification of respiratory complexes was carried out with band densitometry. Graphs show the level of complexes relative to the control samples. Porin was used as a loading control. Red dotted line indicates 50 % of the control samples. Complex activities were measured spectrophotometrically as described in Methods. Data presented in percent changes relative to the control samples. c Citrate synthase activity. Combined data from all three patients shows a significant decrease (p = 0.045) in activity when compared to the control group. d Complex II activity. Combined data from all three patients shows a significant decrease (p = 0.008) in activity when compared to the control group. e Complex IV activity. Combined data from all three patients shows a significant decrease (p < 0.017) in activity when compared to the control group. f mt-DNA copy number. Error bars represent standard deviation. Biliary atresia. g Mitochondrial complexes isolated from control and liver failure patients’ liver were separated using blue native gel electrophoresis on gradient Bis-Tris acrylamide gels. h Quantification of respiratory complexes was carried out with band densitometry. Graphs represent the level of complexes relative to the control samples. Band intensities were normalised to complex II. Red dotted line indicates 50 % of the control samples. i Complex II activity. j Complex IV activity. Error bars represent standard deviation