Table 1.
Variables/characteristics | Biopsy |
Radical prostatectomy |
Total | ||||
---|---|---|---|---|---|---|---|
Contralateral | Adjacent | Tumor | Contralateral | Adjacent | Tumor | ||
Sample storage age, mean (SD), years | 3.29 (2.69) | 3.06 (2.69) | |||||
Available tissue | 24 | 24 | 33 | 23 | 23 | 31 | 158 |
Clinicopathological | |||||||
Tumor content, % | |||||||
Median | 0 | 0 | 70 | 0 | 0 | 80 | |
Range | 0–0 | 0–0 | 10–90 | 0–0 | 0–0 | 50–90 | |
Stromal content, % | |||||||
Median | 60 | 60 | 25 | 60 | 60 | 20 | |
Range | 40–80 | 40–80 | 10–80 | 10–80 | 40–80 | 5–50 | |
Gleason score | |||||||
6 | 0 | 0 | 13 | 0 | 0 | 10 | 23 |
7 | 0 | 0 | 14 | 0 | 0 | 16 | 30 |
8 | 0 | 0 | 4 | 0 | 0 | 4 | 8 |
9 | 0 | 0 | 2 | 0 | 0 | 2 | 4 |
Quality control | |||||||
Successful RNA extraction | 23 | 22 | 27 | 23 | 23 | 31 | 149 |
Failed RNA extraction | 1 | 2 | 6 | 0 | 0 | 0 | |
Median RNA yield, ng | 463.28 | 283.74 | 278.25 | 2814.3 | 2319.04 | 2846.7 | |
Successful cDNA amplification | 23 | 22 | 27 | 22 | 23 | 31 | 148 |
Failed cDNA extraction | 0 | 0 | 0 | 1 | 0 | 0 | |
Median cDNA yield, ng | 7474.68 | 6984.275 | 7066.44 | 6704.88 | 7011.2 | 6461.91 | |
Array good QC | 23 | 22 | 26 | 22 | 23 | 31 | 147 |
Median % present | 54.34 | 51.16 | 48.65 | 46.91 | 51.03 | 49.44 |
The array quality is equivalent across tissue types and samples.
QC, quality control.