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. 2016 Mar;18(2):235–243. doi: 10.1016/j.jmoldx.2015.10.004

Figure 3.

Figure 3

Detection of 0.05% and 0.01% allele frequency of SMAD4 R361G mutation. A: Detection of 0.05% SMAD4 R361G mutation with and without multiplexed preamplification before digital PCR. Water alone [no template control (NTC)], 50 ng wild-type DNA, and 50 ng tumor DNA containing 0.05% mutant allele frequency were either analyzed by digital PCR alone (no preamplification) or preamplified before digital PCR (multiplexed preamplification). Non-preamplified samples (top panels) and samples subjected to multiplexed preamplification (bottom panels) were analyzed for the presence of SMAD4 R361G. B: Detection of 0.01% SMAD4 R361G mutation with multiplexed preamplification before digital PCR. The numbers shown in the bottom right of each plot indicate the number of mutant alleles detected for each condition. P values are denoted in the top right of each plot. Arb., arbitrary.