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. 2016 Apr 30;13:31. doi: 10.1186/s12986-016-0089-7

Table 1.

Primer sequences and real-time PCR conditions used for gene expression analysis by qRT-PCR

Gene name Primer Sequence (5’-3’) AT
I-FABP ATGGAAAGGAGCTGATTGCT 59 °C
TTGGCCTCCACTCCTTCATA
TLR4 GATGCCTCTCTTGCATCTGG 60 °C
TCATGAGGGATTTTGCTGAGA
GAPDH GGCATCGTGGAAGGGCTCATGAC 62 °C
ATGCCAGTGAGCTTCCCGTTCAGC

AT annealing temperature, GAPDH glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene), I-FABP intestinal fatty acid binding protein, TLR4 Toll-like receptor 4