Table 1.
Gene name | Primer Sequence (5’-3’) | AT |
---|---|---|
I-FABP | ATGGAAAGGAGCTGATTGCT | 59 °C |
TTGGCCTCCACTCCTTCATA | ||
TLR4 | GATGCCTCTCTTGCATCTGG | 60 °C |
TCATGAGGGATTTTGCTGAGA | ||
GAPDH | GGCATCGTGGAAGGGCTCATGAC | 62 °C |
ATGCCAGTGAGCTTCCCGTTCAGC |
AT annealing temperature, GAPDH glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene), I-FABP intestinal fatty acid binding protein, TLR4 Toll-like receptor 4