Fig. 4.

The expression of D cyclins in Ccnd1 ^Δ1-3 mice. a Expression levels of Ccnd2, Ccnd3, and exons 4–5 of Ccnd1 relative to the RPII housekeeping gene. Results are from sorted naïve CD8⁺T cells before (upper graphs) or after 24 h stimulation with anti-CD3 mAbs (lower graphs). RPII housekeeping gene expression levels were similar in all mouse Groups. Results are the mean⁺/₋ SD of three independent experiments. Statistic significance: *p < 0.05; **p < 0.005; ***p < 0.001 (paired student t-test). In each experiment amplifications from different Groups were performed simultaneously. b Expression of truncated 4–5 D1 protein by resting CD8⁺ T cells from Group I (upper left panel) and Group III mice (upper right panel); by activated CD8⁺ T cells from group III mice (lower panels): lower right panel show 4–5 migration into the nucleus and lower left panel the interaction with the DNA (in grey) upon T cell activation. The 4–5 D1 fragment was identified with rat anti-mouse D1 4–5 fragment mAb from ProMab Biotechnologies, Richmond, USA, revealed with a goat anti-rat Ab (Molecular Probes). The nucleus was labeled with DAPI and is shown in Red. Co-localization analysis was performed using the Image J program