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. 2016 Apr 30;9:17. doi: 10.1186/s13072-016-0066-4

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© Hainer and Martens. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Single amino acid substitutions expressed at both HHT1-HHF1 and HHT2-HHF2 strongly derepress SER3. Northern blot analysis examining the effect of histone mutants on SER3, SRG1, and SCR1 (loading control). Total RNA was isolated from yeast strains that were grown to a density of 1–2 × 10⁷ cells/mL in media with or without serine at 30 °C. All yeast strains were derivatives of JDY86 expressing either synthetic WT copies of histone H3 at both genomic locations (YS417) or mutants hhts-K122A (YS404), hhts-Q120A (YS409), hhts-R49A (YS428), also expressed at both genomic locations