Fig. 3.

Histone mutations result in decreased Spt2, Spt6, and Spt16, occupancy over highly transcribed, but not lowly transcribed, genes. a ChIP analysis was performed on chromatin prepared from strains expressing HHTS-HHFS alleles (YS454-YS456, YS493-YS495) or the indicated histone mutant alleles (YS458-YS462, YS465, YS471, YS472, YS474, YS504-YS506, YS518, YS519, YS521, YS525-YS527) that were grown in YPD at 30 °C. The amount of immunoprecipitated DNA was determined by qPCR and is shown as a percentage of the input material and represents the mean ± SEM of three biological replicate experiments. Factor occupancy was measured within the coding region of a highly transcribed gene, PYK1. The regions assayed by qPCR are marked with the black bars in the diagram provided for the gene. b Factor occupancy at TUB2, a lowly transcribed gene, was determined as described in a