Fig. 5.

H3 K122A results in a reduced rate of histone reassembly over FMP27. a Diagram depicting the experimental procedure upon repression of GAL1pr-FMP27. b Northern blot analysis examining the effect of WT (YS475) and K122A (YS585) on FMP27 expression during transcription repression where SCR1 serves as a loading control. Rpb3 (c), H3 (d), Spt6 (e), Spt16 (f), and Spt2 (g) ChIP was performed on chromatin isolated from strains containing HHTS-HHFS alleles (YS475, YS477, and YS478) or hhts-K122A mutant alleles (YS585-YS587), expressing GAL1pr-FMP27 that were grown in YPGal at 30 °C to approximately 1 × 10⁷ cells/mL (0′), then repressed by adding glucose and time points were taken, as shown (a). The amount of immunoprecipitated DNA was determined by qPCR as a percentage of the input material normalized to a control region in chromosome V (which is unchanged between WT and K122A) and represents the mean ± SEM of three biological replicate experiments