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. Author manuscript; available in PMC: 2016 Apr 30.
Published in final edited form as: Mol Ther. 2008 Jul 29;16(10):1688–1694. doi: 10.1038/mt.2008.164

Figure 1. Western blotting analysis of p22phox expression in mouse eye.

Figure 1

p22phox expression was detected using western blotting in (a) retinal as well as (b) retinal pigment epithelial (RPE) extracts. Protein extracts from retina and RPE/choroid were isolated by sonication, separated on 12% polyacrylamide gels, and transferred to a nitrocellulose membrane, which was then treated with a polyclonal antibody to p22phox. Antibody antigen complexes were detected using alkaline phosphatase-conjugated anti-rabbit immunoglobulin G antibody, and the filter was developed using NBT/BCIP.