Table 1.

Summary of the performance of IMER and in-solution digestion using complex protein mixture.^a

Protein mixture	Number of proteins groups identified with in-solution digestion	Number of proteins groups identified with IMER	Enzyme immobilized	Database search engine
Mouse serum std	137	106	Trypsin/LysC	Mascot
Yeast lysate	487	445	Trypsin/LysC	PD
Yeast lysate	473	368	LysN	Mascot
Human lysate	207	216	Trypsin/LysC	PD

^aIMER digestion time was 5 min at room temperature. LC-MS/MS analysis for in-solution and IMER digestion was identical and parameters were described in apparatus session with same protein loading amount of 300 ng.