Figure 4.

L-mifamurtide alone or associated with ZA does not directly affect tumor cell proliferation in vivo and in vitro. A. Osteosarcoma cells were treated with ZA, L-mifamurtide or both at indicated doses for 48 h. Cell growth was determined by crystal violet analysis and compared to control untreated cells. B. KHOS tumor biopsies were collected and Ki67 staining was evaluated by immunohistochemical analysis. Specimens were scored and estimated in percentage mean of Ki67⁺-cells compare to the control group, which corresponds to the mice bearing tumor that did not receive any treatment but vehicle. C. Representative picture of Ki67 staining for control and bitherapy treated groups.