Palmitate rescue experiments. 3T3-L1 cells were treated with SA for 8 days with or without exogenous palmitate and stained with ORO (A). Expression of PPARγ mRNA (B), FABP4 mRNA (C), and FABP4 protein (representative blot, D). *P<0.05, **P<0.01, ****P<0.0001 compared to vehicle control; n=4 for lipid accumulation experiments; n=3 for mRNA and protein expression studies.