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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: Eur J Pharmacol. 2016 Mar 31;780:202–208. doi: 10.1016/j.ejphar.2016.03.052

Figure 4.

Figure 4

Rosiglitazone rescues adipogenic gene expression despite treatment with SA. Cells differentiated with a rosiglitazone/insulin cocktail were treated with either a DMSO vehicle control, or 100 nM SA with or without 200 µM palmitate. A) Cells were fixed at day 8 and stained with ORO and absorbance was measured at 490 nm. Gene expression of (B) PPARγ and (C) FABP4 was measured at 8 days after induction of differentiation. D) Representative western blot image of FABP4 protein expression. (One-way ANOVA; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 compared to vehicle control; n=4 for lipid accumulation experiments; n=3 for gene and protein expression studies).