Figure 3.

4-PI induced growth inhibition can be restored after removing the inhibitor or adding exogenous cholesterol. Chlamydia trachomatis infected cells with or without 1 mM 4-PI were incubated for 24 h and the medium exchanged with inhibitor, with inhibitor and 25 μg ml⁻¹ cholesterol, or medium without inhibitor and further incubated for additional 24 h. Cells were harvested 48 h p.i. and HeLa cells fresh infected with dilutions of the harvested samples to titrate the C. trachomatis IFU. All titrations are the mean of three biologically independent replicate samples. Error bars represent SD.