Figure 5.

Lipids in serum are the main contributing components during recovery of C. trachomatis after 4-PI growth inhibition. HeLa cells were left untreated or pre-treated with 1 mM 4-PI in serum-free medium and infected with an MOI of 1. (A) After 24 h, the medium was replaced with fresh medium with or without supplements of cholesterol, or pools of vitamins, salts or lipids, respectively. (B) After 24 h the medium was replaced with fresh medium with or without supplements of glyceride trilinoleate, phosphocholine, cholesterol, linoleic acid, cholesteryl linoleate or combinations of the latter three. Cells were harvested 48 h p.i. and HeLa cells fresh infected with dilutions the harvested samples to titrate the C. trachomatis IFU. All titrations are the mean of three biologically independent replicate samples and error bars represent SD.