Figure 7.

Chlamydial growth is reduced by the specific ACAT inhibitor and can be restored with cholesteryl linoleate. HeLa cells were left untreated or pre-treated with either 1 mM 4-PI or 200 μM ACAT inhibitor. HeLa cells were infected with an MOI of 1 and after 24 h p.i. the inhibitor containing medium replace with 25 μg ml⁻¹ cholesteryl linoleate. The cells were harvested after 48 h p.i. and fresh HeLa cells were infected with dilutions of harvested samples to titrate the C. trachomatis IFU. All titrations are the mean of three biologically independent replicate samples and error bars represent SD.