Table 1.
Gene primers sequence and annealing temperature
| Gene | GeneBank | (5′→3′) | (Tm, °C) | (bp) |
|---|---|---|---|---|
| GAPDH | NM_001206359.1 | Forward: GATGGTGAAGGTCGGAGTGAAC Reversed: TGGGTGGAATCATACTGGAACA |
60.9 | 153 |
| Occludin | NM_001163647.2 | Forward: ACGAGCAGCAAAGGGATTCTTC Reversed: TCACACCCAGGATAGCACTCATT |
61.2 | 152 |
| Claudin-1 | NM_001244539.1 | Forward: TGCCTCAGTGGAAGATTTACTCC Reversed: TGGTGTTCAGATTCAGCAAGGA |
60.1 | 147 |
| ZO-1 | XM_005659811.1 | Forward: AGTTTGATAGTGGCGTTGACAC Reversed: GCTGAAGGACTCACAGGAACA |
60.0 | 106 |
| Akt | NM_001159776.1 | Forward: GCACAAACGAGGCGAGTA Reversed: CAGCGGATGATGAAGGTGTT |
61.4 | 195 |
| m-TOR | XM_003127584.4 | Forward: GGATGCTGGTGTCCTTTGTGA Reversed: TGCTCTGGATGGAGGTGTTCAT |
60.09 | 102 |
Tm, temperature; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ZO-1, zonula occludens protein 1; m-TOR, mammalian target of rapamycin.