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. 2016 May 2;7:630. doi: 10.3389/fmicb.2016.00630

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Copyright © 2016 Guo, Tan, Nie, Zhu, Pan and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of MoPmt2 on mycelia growth of M. oryzae. (A) Mycelial growth is altered in the MoPmt2 mutant. Colony diameters of the tested strains on different media were measured and statistically analyzed by Duncan analysis. (B) Phenotype of mycelia grown in liquid CM. All tested strains were inoculated in liquid CM for 48 h at 28°C in darkness and then photographed. (C) Measurement of fungal biomass. The fungal mycelia of the tested strains, grown in liquid CM for 3 and 5 days respectively, were collected and freezing dried, and were statistically analyzed. (D) Mycelia growth on water agar media. CFW staining of mycelia is used to show the distance of septa and swollen of mycelia. Asterisks in white color indicate septa. (E) The average length of the sub-apical cell of vegetative hyphae in Guy11, MoPmt2-6, MoPmt2-8, and MoPmt2c. The mean and standard deviations were calculated based on three independent experiments by measuring at least 50 sub-apical cells in each replicate. Error bar represents standard deviation, and asterisks in this figure indicate significant differences from the control (P < 0.01).