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Preparation of partially deproteinized bone.
| Reagent/treatment | Processing time | Temperature |
|---|---|---|
| 30% H2O2 | 72 h∗ | RT |
| Distilled water | 30 min | RT |
| Ethanol | 24 h | RT |
| Distilled water | 30 min | RT |
| Acetone | 24 h | RT |
| Distilled water | 30 min | RT |
| Dying oven | 8 h | RT |
∗30% H2O2 is changed every 24 h during 72 h.
RT: room temperature.
