Fig. 2.

A. Primary hepatocytes were maintained in a system model based on the sinusoidal configuration [29] under conditions that maintain the physiological hemodynamics and transport, and shown to retain and restore liver like phenotype, morphology, function and responses. B. An H&E stain of the liver from the propionic acidemia patient used in this study, reveals normal in vivo morphology. Hepatocytes isolated from the propionic acidemia patient and cultured in the system demonstrate retention of polarized morphology (Green — Phalloidin, Blue — Draq 5) that is stable over time (C. — 4 days, D. — 7 days, E. — 10 days).