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. 2016 Apr 30;16:191. doi: 10.1186/s12879-016-1523-1

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© Chaves et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The apoptotic profile of T lymphocytes from peripheral blood of uninfected individuals (NI) and patients with Chagas disease - indeterminate (IND) or cardiac (CARD) form. The detection of apoptosis was performed using the percentage of annexin⁺ (panel a) and caspase 3⁺ (panel b) in total lymphocytes, CD4⁺ or CD8⁺ T cell subsets. The lymphocytes were evaluated without stimulation - control cultures (C) and after in vitro stimulation with TcAg (T. cruzi antigens) and STP (staurosporin). Mann–Whitney test was used for comparison and the results were expressed as the median with interquartile range. Differences (p < 0.05) are presented by the corresponding numbers and connecting lines in the box plot graphs