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. 2016 Apr 30;7:68. doi: 10.1186/s13287-016-0324-1

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© Liu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Contribution of BrdU⁺ LRCs in the regeneration of I/R injured mouse kidneys. a Double-immunofluorescence staining for BrdU (red) and Ki67 (green) to visualize the role of BrdU⁺ LRCs in cell proliferation 4 days after IRI. Newly generated cells formed a special niche-like structure (green), and only some of the BrdU⁺ LRCs co-expressed Ki-67 in this area (arrows). b Double immunofluorescence was used to determine BrdU⁺ LRCs undergoing cellular apoptosis on day 4. Scattered BrdU⁺ LRCs co-expressed TUNEL (arrows) (arrows indicate the double-stained location; magnification × 400). BrdU 5-bromo-2'-deoxyuridine, LRCs label-retaining cells, I/R ischemia/reperfusion