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. 2016 Apr 6;6(4):150164. doi: 10.1098/rsob.150164

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — Effect of SepG on the localization of eGFP fusions of SsgA, SsgB and FtsZ. All proteins localize correctly in wild-type cells (M145, left). In contrast, SepG is required for the formation of ladders of FtsZ or for the localization of SsgB at division sites. SsgA has a normal focal pattern in the sepG mutant, whereas focal patterns for SsgB were seen in around 33% of the aerial hyphae (67% of the GAL1 aerial hyphae showed diffuse localization of SsgB); SsgB fails to localize to the future septum sites in sporogenic aerial hyphae of GAL1. The occasional Z rings formed in sepG null mutant GAL1 are also seen in ssgB mutants [27]. Cultures were grown on SFM agar plates for 5 days at 30°C. Scale bar, 1 µm (for all images).