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. 2016 Apr 6;6(4):150164. doi: 10.1098/rsob.150164

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 8. — Membrane topology of SepG. (a) Growth of E. coli JM109 carrying plasmids expressing different BlaM fusions, namely full-length SepG–BlaM (pGWS793); N-terminal SepG_1-40-BlaM (pGWS794; first TM domain of SepG); C-terminal SepG_41–94-BlaM (pGWS795; second TM domain of SepG); BlaM with signal sequence expressed from the ftsZ promoter(s) (pGWS796; positive control); BlaM with signal sequence expressed from its native promoter (pHJL401; positive control); and pGWS755 expressing SepG–eGFP (pGWS755; negative control). Transformants were grown overnight at 37°C on LB agar plates with 0, 10 or 20 µg ml⁻¹ ampicillin. (b) Topological model of SepG. Left, wild-type SepG, with the topology model generated using TMRPRES2D [47]. Right, model of SepG fused to eGFP, drawn based on the wild-type SepG model.