FIGURE 1.

A flowchart showing different stages of trait discovery and trait deployment using NGS approaches for achieving higher genetic gains and accelerated development of improved genotypes. The different germplasm sets (such as core collection, reference set, and mutants) and genetic populations (RILs, NAM, MAGIC, and AB-QTL) provide genetic variation for agronomically important traits. These populations are subjected to next generation sequencing and high-throughput genotyping, and high quality phenotyping leading to the discovery of candidate genes and trait specific diagnostic markers. Such markers are prerequisite for deploying MAS, MABC, and MARS in crops. Different “omics” approaches can play an important role in achieving better understanding and greater insights for the target traits. In addition, the deployment of genomic selection breeding will help in achieving higher genetic gains in less time.