Esrp1, Sostdc1, Fermt1, Trim29, Tmprss2 and Lamc2 expression is reduced in Grhl2 mutant embryos. (A) qRT-PCR analysis shows decreased expression of NNE genes in Grhl2 mutant versus wild-type embryos. Experiments were performed in biological quadruplicate with technical quintuplicates. Mean±s.d. *P<0.05, **P<0.01, Student's t-test. (B) In situ hybridization of transverse sections of cranial neural folds shows altered expression of Esrp1, Fermt1, Trim29, Tmprss2 and Lamc2 in Grhl2 mutant NNE. Four embryos per genotype were analyzed in separate technical replicates.