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. 2016 Apr 1;143(7):1192–1204. doi: 10.1242/dev.129825

Fig. 5.

Fig. 5.

Loss of Grhl2, Esrp1, Sostdc1, Fermt1, Tmprss2 or Lamc2 in vitro results in a mesenchymal phenotype. (A) qRT-PCR analysis of RNA from IMCD-3 cells shows strong expression of Grhl2 and its targets relative to Grhl3. (B,C) Control KD IMCD-3 (shControl) cells have an epithelial cobblestone morphology, whereas Grhl2 KD (shGrhl2) cells appear mesenchymal and show a significant decrease in expression levels of Grhl2 and its targets as assessed by qRT-PCR. (A,C) Experiments were performed in biological quadruplicate with technical quintuplicates. Mean±s.d. **P<0.01, ***P<0.001, Student's t-test. (D) Cells subject to individual gene KD for Esrp1, Sostdc1, Fermt1, Tmprss2 or Lamc2 exhibit a mesenchymal morphology.