Skip to main content
. 2016 Apr 1;143(7):1192–1204. doi: 10.1242/dev.129825

Fig. 6.

Fig. 6.

Loss of Grhl2, Esrp1, Sostdc1, Fermt1, Tmprss2 or Lamc2 expression in IMCD-3 cells causes altered protein expression indicative of an EMT. Immunofluorescence for ZO-1 and vimentin (A-G) or E-cadherin and N-cadherin (H-N) on stable KD cell lines. (A) shControl cells exhibit a highly regular pattern of ZO-1 at the tight junctions between cells, and do not express vimentin. (B) shGrhl2, (C) shEsrp1, (D) shSostdc1, (E) shFermt1, (F) shTmprss2 and (G) shLamc2 cells all show irregular ZO-1 and aberrant vimentin expression patterns. (H) shControl cells exclusively express E-cadherin at the adherens junctions. (I) shGrhl2, (J) shEsrp1, (K) shSostdc1, (L) shFermt1, (M) shTmprss2 and (N) shLamc2 cells all show a switch from E-cadherin to N-cadherin expression. Experiments were performed in triplicate. Scale bars: 20 µm.