Fig. 7.

Grhl2, Esrp1, Sostdc1, Fermt1, Tmprss2 and Lamc2 KD cells undergo a functional EMT. (A) Images of whole Transwell filters seeded with IMCD-3 cells in which the specified genes have been subject to KD by shRNA, showing their differential ability to migrate toward a chemoattractant. (B) Quantification of A. (C) Quantification of the total number of KD cells that invaded through a basement membrane relative to migration through the filter alone. Experiments were performed in biological and technical triplicate. Mean±s.d. *P<0.05, **P<0.01, ***P<0.001, Student's t-test.