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. 2016 Apr 1;143(7):1134–1148. doi: 10.1242/dev.131060

Fig. 3.

Fig. 3.

Tctp is not necessary for the timely development of the eye. (A) Representative stage 43 control and Tctp-depleted retinas stained with phalloidin and DAPI. The boxed areas are shown at a higher magnification to the right. (B,C) Immunohistochemistry analysis of the photoreceptor layer in stage 43 wild-type retinas probed with anti-Tctp and anti-opsin or anti-rhodopsin antibodies, and counterstained with DAPI. IS, photoreceptor inner segment; ONL, outer nuclear layer; OS, photoreceptor outer segment; PR, photoreceptor. (D,E) Representative micrographs of the photoreceptor layer in stage 43 control or Tctp morphant retinas probed with anti-opsin or anti-rhodopsin antibodies, and counterstained with DAPI. (F) Average inner segment lengths in control and Tctp morphant retinas. ***P<0.0001, unpaired t-test; box plot whiskers denote 5th-95th percentile. (G) Proportion of photoreceptors showing a complete loss of the outer segment in control and Tctp morphant retinas. n, number of photoreceptor layers analysed; ***P<0.0001, unpaired t-test. Scale bars: 50 μm in A; 25 μm in B-E.