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. 2016 Apr 15;143(8):1271–1283. doi: 10.1242/dev.131961

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Upregulation of BAF155 causes upregulation of BAF complex components. (A) HA-tagged human BAF155, mouse Baf57 or Ruby mRNAs were injected into one blastomere at the 2-cell stage and analysed at the 8-cell stage. (B-B″) Clonal overexpression of BAF155 results in the upregulation of protein levels of the complex subunits (B′), whereas overexpression of Ruby (B) or BAF57 (B″) does not. (C) The protein levels of BAF57 and BRG1 upon BAF155 overexpression (OE) were upregulated by ∼3-fold. (D) qRT-PCR analysis of transcripts for key components of the BAF complex 24 h after BAF155 OE. INI1 refers to Baf47 (Smarcb1). (E) HA-tagged BAF155 mRNA was injected into one blastomere at the 2-cell stage and analysed at the 4-cell stage by PLA. (F) Clonal BAF155 OE caused an increase in BAF155-BRG1 interaction in the injected clones (dashed outline). (G) Overexpression of exogenous BAF155 resulted in 2-fold upregulation of BAF155-BRG1 contact. Error bars represent s.d. *P<0.05, **P<0.01, ***P<0.001, Student's t-test.