Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 15;143(8):1271–1283. doi: 10.1242/dev.131961

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 6. — NANOG⁺ cells contain more mobile BAF155 than NANOG⁻ cells. (A) Rescue conditions in Nanog-YFP transgenic embryos were applied to measure the live kinetics of BAF155 between the lineages at E4.5. The kinetics of CENPA were measured in embryos injected with control RNAi at the zygote stage (n=11). (B,C) The recovery kinetics of BAF155 and CENPA were assessed in a rectangular region of nuclei after photobleaching. The fluorescence intensity was measured prior to photobleaching (P) and for 40 s during the recovery phase after photobleaching (0 s). (D) NANOG⁺ cells show greater recovery of BAF155 than NANOG⁻ cells, whereas recovery of CENPA is similar. (E) NANOG⁻ cells have a significantly higher immobile fraction of BAF155 protein (87.7±4.3%) than NANOG⁺ cells (72.06±6.2%); *P<0.05, F-test. The difference in the size of the immobile fraction of CENPA between NANOG⁻ (95.17±1.2%) and NANOG⁺ (96.31±3.6%) cells was not statistically significant (P>0.05, F-test). Error bars represent s.e.m.